But, the therapeutic option for patients meeting the Milan criteria with preserved liver function is facing a dilemma. In this study, we examined the outcomes of surgical resection for HCC patients meeting the Milan criteria with preserved liver function and rationale of hepatic resection as the first treatment for HCC meeting the Milan Criteria.\n\nMethods: Between 1991 and 2006, 248 patients with HCC underwent hepatectomy in 1.58 primary HCC patients meeting Milan criteria (Group M) and in 90 patients beyond Milan criteria (Group N). Median age was 54.5 years in group M and 52.4 years in group N.\n\nResults:

The tumor size, mean survival months, E-S grade and vascular invasion rate were significantly different between the two groups. 67 patients in group M and 22 patients in group N had intrahepatic recurrence selleck chemicals after primary hepatectomy.

The cumulative 1, 3, and 5-year survival rates were 91.9%, 74.5%, and 60.5% in intrahepatic recurrence group M after primary hepatectomy and 100%, 96.0%, and 90.5% in repeated hepatic resection for recurrent treatment in group M, respectively.\n\nConclusion: Because of the high survival rate and long-term Survival after adequate treatment of recurrence, primary hepatectomy is considered a reasonable option as first-line treatment for HCC meeting Milan criteria with preserved liver function. (J Korean Surg Soc 2009;77:267-272)”
“Aim Understanding find more the factors underlying the successful establishment of invasive ant species is critical for developing quarantine strategies to prevent additional invasions as well as for determining how such species overcome the selective pressures in invaded areas. Although several studies have revealed differences in the social organization and population genetics of

invasive ants in their native and introduced ranges, few studies have considered the potential interactions between alternate social forms within newly-invaded areas simply because many invasive ants are characterized as polygyne or unicolonial.\n\nLocation Taoyuan and Chiayi County, Taiwan.\n\nMethods Both social forms (polygyne and monogyne) of the red imported fire ant, Solenopsis invicta, occur in two separately invaded areas in Taiwan (Taoyuan and Chiayi). We DMH1 order employed intensive sampling methods and diagnostic polymerase chain reaction assays to determine the distribution of these two social forms in both infested areas in Taiwan.\n\nResults The distribution of social forms differs dramatically between the two infested areas, consistent with different invasion histories regarding the original make-up of individuals comprising the initial founder group. The Taoyuan population likely was colonized initially by ants of both social forms, with the subsequent spread characterized by continuous outward movement of the two forms, particularly the monogyne form.

Potential exposure to risk seemed to be important for patient expectations. Conclusions: Paternalism seemed to dominate our clinical setting. OSI-744 Protein Tyrosine Kinase inhibitor The informed-consent process was definitely a separate issue from signing the consent forms. We conclude that the informed-consent process should be modified to be more functional and appropriate to human psychology. We suggest that education

is necessary for informed consent to promote better quality and safety in health care.”
“Curcumin, a compound found in the Indian Spice tumeric, has anti-inflammatory and immunomodulatory properties, though the mechanism remains Unclear. Dendritic cells (DCs) are important to generating an immune response and the effect of curcumin on human DCs has not been explored. Selleckchem CHIR 99021 The role curcumin in the DC response to bacterial and vital infection was investigated in vitro using LPS and Poly I:C as models of infection. CD14(+) monocytes, isolated from human peripheral blood,

were cultured in GM-CSF- and IL-4-supplemented medium to generate immature DCs. Cultures were incubated with curcumin, stimulated with LPS OF Poly I:C and functional assays were performed. Curcumin prevents DCs from responding to immunostimulants and inducing CD4(+) T cell proliferation by blocking maturation marker, cytokine and chemokine expression and reducing both migration and endocytosis. These data Suggest a therapeutic role for curcumin as an immune suppressant. Published by Elsevier Inc.”
“Micelles formed in the GI tract by bile acid and lecithin play an important role in oral absorption of poorly soluble drugs. In this situation, the drug molecules are present in equilibrium between the free and micellar states. In this study, the relationship between the free drug concentration and the membrane permeability of poorly soluble drugs was examined. Permeability across a Caco-2 monolayer and a dialysis membrane were measured in a side-by-side chamber system. The concentrations of sodium taurocholate (NaTC) and lecithin were varied to allow measurement of membrane permeability

at different concentrations of free drugs. For troglitazone, hexylparaben, and heptylparaben, an increase in the NaTC and lecithin concentrations caused the permeability across the Caco-2 monolayer to decrease slightly, whereas the permeability across the Screening Library dialysis membrane decreased markedly. In contrast, the changes in permeability of griseofulvin with an increased micelle concentration were similar for the Caco-2 monolayer and the dialysis membrane. Assuming that the permeability for the dialysis membrane reflects the free drug concentration in the medium, these results suggest that troglitazone and alkylparabens, but not griseofulvin, can partition directly from micelles to Caco-2 monolayers. This mechanism may contribute to oral absorption of drugs that are poorly soluble in water. (C) 2009 Wiley-Liss, Inc.

Functionally competent CD8(+) T cells specific against tumor antigens (e.g. Her2/neu and MAGEA3) as well as against viral antigens have been recently generated from cord blood mononuclear SHP099 cells suggesting that cord blood can be a source of “young” anti-tumor T cells for adoptive

cancer immunotherapy. Moreover, cord blood can give rise to antigen non-specific effector cells including NK cells and dendritic cells. Finally, umbilical cord blood anti-tumor specific T cell clones are unlikely to have participated in tumor immunoediting, making them more efficient than host T cells in eradicating tumor cells. (C) 2010 Elsevier Ireland Ltd. All rights reserved.”
“Deconvolution of the role of off-cycle species from the desired catalytic cycle leads to an optimized protocol for the prolinate-catalyzed amination of aldehydes. The scope of complex reaction networks will be greatly broadened by understanding ancillary rate processes that influence the productive catalytic pathway.”
“Background: Nucleoli are composed of possibly several thousand different proteins and represent the most conspicuous compartments in the nucleus; they play a crucial role in the INCB028050 order proper execution of many cellular processes. As such, nucleoli carry out ribosome biogenesis and sequester

or associate with key molecules that regulate cell cycle progression, tumorigenesis, apoptosis and the stress response. Nucleoli are dynamic compartments that are characterized by a constant flux of macromolecules. Given the complex GSK1838705A and dynamic composition of the nucleolar proteome, it is challenging

to link modifications in nucleolar composition to downstream effects.\n\nResults: In this contribution, we present quantitative immunofluorescence methods that rely on computer-based image analysis. We demonstrate the effectiveness of these techniques by monitoring the dynamic association of proteins and RNA with nucleoli under different physiological conditions. Thus, the protocols described by us were employed to study stress-dependent changes in the nucleolar concentration of endogenous and GFP-tagged proteins. Furthermore, our methods were applied to measure de novo RNA synthesis that is associated with nucleoli. We show that the techniques described here can be easily combined with automated high throughput screening (HTS) platforms, making it possible to obtain large data sets and analyze many of the biological processes that are located in nucleoli.\n\nConclusions: Our protocols set the stage to analyze in a quantitative fashion the kinetics of shuttling nucleolar proteins, both at the single cell level as well as for a large number of cells. Moreover, the procedures described here are compatible with high throughput image acquisition and analysis using HTS automated platforms, thereby providing the basis to quantify nucleolar components and activities for numerous samples and experimental conditions.

SWNHs infused at a rate of 50 mu L/min resulted in an average lateral expansion rate of 0.36 +/- 0.08 cm(2)/min. Infused SWNHs dispersal depth was limited to the urothelium and muscular propria for 50 mu L/min infusions

of 10 min or less, but dispersed through the entire thickness after a 15-min infusion period. Irradiation of SWNH-perfused bladder tissue with 1,064 nm laser light at 0.95 W/cm(2) over 40 s exhibited a maximum increase of approximately 19 A degrees C compared with an increase of approximately 3 A degrees C in a non-perfused control. learn more The results indicate that these silica FMDs can successfully penetrate into the bladder wall to rapidly distribute SWNHs with some degree of lateral and depth control and Stem Cell Compound Library high throughput that SWNHs may be a viable exogenous chromophore for photothermal amplification of laser-based UCC treatments.”
“Background: The purpose of this study was to evaluate changes in subfoveal choroidal thickness after photodynamic therapy in patients with acute idiopathic central serous chorioretinopathy (ICSCR).\n\nMethods: This was a retrospective observational study conducted in 63 participants. The primary outcome measure was subfoveal choroidal thickness at baseline and 3 days,

one week, 4 weeks, and 12 weeks after photodynamic therapy. The secondary outcome measure was indocyanine green angiography at baseline and 4 weeks and 12 weeks after photodynamic therapy.\n\nResults: Four weeks after photodynamic therapy, 20 (64.51%) symptomatic eyes showed hypofluorescence corresponding to the area of photodynamic therapy irradiation at the posterior pole. The mean subfoveal choroidal thickness increased significantly from 422 +/- 132 mu m at baseline to 478 +/- 163 mu m at day 3 after treatment (P=0.022) and then decreased to 362 +/- 113 mu m at week 4 (P<0.001) and 339 +/- 135 mu m at week 12 (P<0.001).\n\nConclusion: The subfoveal choroid in patients with acute ICSCR is thicker than in the normal population, and in symptomatic eyes is significantly thicker than in fellow eyes. Photodynamic therapy using a one third dose of verteporfin may decrease

choroidal vascular CX-6258 hyperpermeability and choroidal thickness in patients with acute ICSCR.”
“Objectives. Diabetes mellitus (DM) in combination with heart failure (HF) is associated with a high risk of death, but it is uncertain whether the prognosis differs in ischemic and non-ischemic HF groups. Design. One thousand, three hundred and six patients with ischemic HF and 1315 patients with non-ischemic HF were followed for 6.8 years. Risk of all caue mortality was analyzed using Cox proportional hazard models. Results. Mean age was 73 (+/- standard deviation 11) years. Two hundred and forty eight of 1306 (19%) patients with ischemic HF and 172/1315 (13%) patients with non-ischemic HF had DM (p for difference < 0.0001). Overall, 939/1306 (72%) patients in the ischemic HF group, and 835/1315 (64%) patients in the non-ischemic HF group died, respectively.

Kaplan-Meier curves were used to estimate the association between CIP2A expression and patient survival. The functional role of CIP2A https://www.selleckchem.com/products/sbi-0206965.html in NPC cell lines was evaluated by small interfering RNA-mediated depletion of the protein followed by analyses of cell proliferation and xenograft growth. Results: CIP2A levels were upregulated in NPC cell lines and clinical samples at both the mRNA and protein levels (P smaller than 0.01). Patients with high CIP2A expression had poorer overall survival (HR, 1.98; 95% CI, 1.16-3.34; P = 0.01) and poorer disease-free survival (HR, 1.68; 95% CI, 1.07-2.62; P = 0.02) rates than patients with low CIP2A expression. In addition,

CIP2A expression status was an independent prognostic indicator for NPC patients. The depletion of CIP2A

expression inhibited c-Myc protein expression in NPC cell lines, suppressed cell viability, colony formation, and anchorage-independent growth in vitro, and inhibited xenograft tumor growth in vivo. Conclusions: AS1842856 Our data demonstrate that high CIP2A expression in patients was associated with poor survival in NPC, and depletion of CIP2A expression inhibited NPC cell proliferation and tumor growth. Thus, these results warrant further investigation of CIP2A as a novel therapeutic target for the treatment of NPC.”
“Rheumatoid arthritis (RA) is a chronic inflammatory disease which leads to destruction of joints. Current treatment modalities for RA either produce symptomatic relief (NSAIDs) or modify the disease process (DMARDs). Though effective, their use is also limited by their side effects. As a result, the interest in alternative, well tolerated anti-inflammatory remedies has re-emerged. Our aim was to evaluate the antioxidant and antiarthritic activity of Boswellia serrata gum resin extract (BSE) in collagen induced arthritis. Arthritis was induced in male Wistar rats by collagen induced arthritis (CIA) method. BSE was administered at doses of 100 and 200 mg/kg

body weight once daily for Volasertib 21 days. The effects of treatment in the rats were assessed by biochemical (articular elastase, MPO, LPO, GSH, catalase, SOD and NO), inflammatory mediators (IL-1 beta, IL-6, TNF-alpha, IL-10, IFN-gamma and PGE(2)), and histological studies in joints. BSE was effective in bringing significant changes on all the parameters (articular elastase, MPO, LPO, GSH, catalase, SOD and NO) studied. Oral administration of BSE resulted in significantly reduced levels of inflammatory mediators (IL-1 beta, IL-6, TNF-alpha, IFN-gamma and PGE(2)), and increased level of IL-10. The protective effects of BSE against RA were also evident from the decrease in arthritis scoring and bone histology. The abilities to inhibit proinflammatory cytokines and modulation of antioxidant status suggest that the protective effect of Boswellia serrata extract on arthritis in rats might be mediated via the modulation of immune system.

Compared with control pregnancies, examination of E18.5 placentas arising from TCDD-exposed males on the standard diet revealed a significant increase in Tlr4 mRNA expression corresponding to a reduction in Pgr mRNA and PGDH protein expression. In contrast, fish oil supplementation of toxicant-exposed males led to normalization of placental expression of both Pgr and Tlr4 mRNA and a marked increase in PGDH expression. These studies suggest that a

paternal preconception diet that GS-1101 order includes omega-3 fatty acids prevents the toxicant-associated increase in the placental inflammatory response at late gestation, preventing PTB. Reproduction (2011) 142 235-241″
“Objective-To determine the effect of large colon ischemia and reperfusion on concentrations of the inflammatory neutrophilic protein calprotectin and other clinicopathologic variables in jugular and colonic venous blood in horses.\n\nAnimals-6 healthy horses.\n\nProcedures-Horses were anesthetized, and ischemia was induced for 1 hour followed by 4 hours of reperfusion in a segment of the pelvic flexure of the large colon. Blood samples were obtained before anesthesia, before induction of ischemia, 1 hour after the start of ischemia, and 1, 2, and 4 hours after the start of reperfusion from jugular veins and veins of the segment of the large colon that underwent ischemia and reperfusion. A sandwich ELISA

was developed for detection of equine calprotectin. click here Serum calprotectin concentrations and values of blood gas, hematologic, and biochemical analysis variables were determined.\n\nResults-Large colon ischemia caused metabolic acidosis, a significant increase in lactate and potassium concentrations and creatine kinase activities, and a nonsignificant decrease NVP-LDE225 chemical structure in glucose concentrations in colonic venous blood samples. Values of these variables after reperfusion were similar to values before ischemia. Ischemia and reperfusion induced activation of an inflammatory response characterized by an increase in neutrophil cell turnover rate in jugular and colonic venous blood samples and calprotectin concentrations in colonic venous blood

samples.\n\nConclusions and Clinical Relevance-Results of this study suggested that large colon ischemia and reperfusion caused local and systemic inflammation in horses. Serum calprotectin concentration may be useful as a marker of this inflammatory response.”
“Objectives: Research has provided us with an increased understanding of nociception-motor interaction. Nociception-motor interaction is most often processed without conscious thoughts. Hence, in many cases neither patients nor clinicians are aware of the interaction. It is aimed at reviewing the scientific literature on nociception-motor interaction, with emphasis on clinical implications.\n\nMethods: Narrative review.\n\nResults: Chronic nociceptive stimuli result in cortical relay of the motor output in humans, and a reduced activity of the painful muscle.

“
“The pursuit of ever higher field strengths and faster data acquisitions has led to the construction of coil arrays with high numbers of elements. With the sensitivity encoding (SENSE) technique, it has been shown that the sensitivity of those elements can be used for spatial image encoding. Here, a proof-of-principle is presented of a method that can be considered an extreme case of the SENSE approach, completely abstaining from using encoding gradients. The resulting sensitivity encoded free-induction decay (FID) data GSK3326595 research buy are then not used for imaging, but for determining B-0 field inhomogeneity distribution.

The method has therefore been termed “SENSE shimming” (SSH). In phantom experiments the method’s ability to detect inhomogeneities of up to the second order is demonstrated. Magn Reson Med 62:1319-1325, 2009. (C) 2009 Wiley-Liss, Inc.”
“Premise of the study : Seabirds often cause significant changes

to soil properties, and seabird-dominated systems often host unique plant communities. This study experimentally (1) examined species-specific responses to seabird guano gradients, (2) considered Adavosertib the role that differential functional traits among species play in altering plant response to guano, and (3) investigated the implications of seabird guano on range-expanding species.\n\nMethods : Using a greenhouse fertilization experiment, we examined how guano fertilization affects the growth and functional traits of four tree species dominant in the Pacific Islands: Cocos nucifera, Pisonia grandis, Scaevola sericea, and Tournefortia argentea. In these systems, seabirds are frequently found in association with three of these four species; the remaining species, C. nucifera, is a recently proliferating species commonly found in the region but rarely associated with seabirds.\n\nKey results : We determined that responses to guano addition differed significantly between species in ways that were consistent with predictions based on differing functional traits among species. Notably, we demonstrated that C. nucifera showed no growth responses to guano additions, whereas all seabird-associated plants showed strong responses.\n\nConclusions

: These results provide experimental evidence of differential species find more response to guano additions, suggesting that differences in species functional traits may contribute to changes in plant communities in seabird-dominated areas, with seabird-associated species garnering performance advantages in these high-nutrient environments. Among these species, results also suggest that C. nucifera may have a competitive advantage in low-nutrient environments, providing an unusual example of how a range-expanding plant species can profit from low-nutrient environments.”
“The start of the cholera epidemic in Haiti quickly highlighted the necessity of the implementation of an Alert and Response (A&R) System to complement the existing national surveillance system.

The nonisothermal measurements show double demixing peaks in both the heat flow and the heat capacity traces for dispersions with a weight fraction of Au-PNIPAM (f(w)) below 50 wt %. The lower phase transition corresponds to an inner layer of PNIPAM segments on the surface of the gold core, while the upper transition corresponds to an outer layer. The dispersions follow a lower critical solution temperature phase behavior with a threshold Selisistat demixing temperature at about 11.4 degrees C for f(w) of 45.3 wt %. Comparison with aqueous solutions of PNIPAM with different molar masses shows that

the gold core reduces the miscibility of PNIPAM with water. The process kinetics throughout the phase transition is studied by quasi-isothermal heat capacity measurements, through the effect of the modulation frequency, by changing the heating rate and repeated heating-cooling cycles. Overall, the response for Au-PNIPAM dispersions is markedly faster than for PNIPAM solutions, which might be the result

of water remaining Finely dispersed within the polymer matrix. Close to the gold core, the restricted collapse of the PNIPAM chains, as a result of steric hindrance by neighboring LB-100 molecular weight anchored chains, might result in the presence of water inside the collapsed nanoparticle. Notwithstanding the occurrence of partial vitrification in the polymer-rich phase during heating, the Au-PNIPAM dispersions retain their thermoresponsive behavior after repeated heating-cooling cycles.”
“The flattop buy AZD6094 mountains (tepuis) of South America are ancient remnants of the Precambrian Guiana Shield plateau. The tepui summits, isolated by their surrounding cliffs that can be up to 1000 m tall, are thought

of as islands in the sky, harboring relict flora and fauna that underwent vicariant speciation due to plateau fragmentation. High endemicity atop tepui summits support the idea of an ancient Lost World biota. However, recent work suggests that dispersal between lowlands and summits has occurred long after tepui formation indicating that tepui summits may not be as isolated from the lowlands as researchers have long suggested. Neither view of the origin of the tepui biota (i.e., ancient vicariance vs. recent dispersal) has strong empirical support owing to a lack of studies. We test diversification hypotheses of the Guiana Shield highlands by estimating divergence times of an endemic group of treefrogs, Tepuihyla. We find that diversification of this group does not support an ancient origin for this taxon; instead, divergence times among the highland species are 25 Ma. Our data indicate that most highland speciation occurred during the Pliocene. Thus, this unparalleled landscape known as The Lost World is inhabited, in part, not by Early Tertiary relicts but neoendemics.”
“To study genetic evolution of Moroccan influenza A(H1N1)pdm09 virus strains, we conducted a molecular characterization of the hemagglutinin gene subunit 1 (HA1) of 36 influenza A(H1N1)pdm09 virus strains.

Most soils did not contain detectable concentrations of either myo- or scyllo-inositol hexakisphosphate, which is in marked contrast to many temperate mineral soils that contain abundant inositol phosphates. We conclude that soil properties exert a strong control on the amounts and forms of soil organic phosphorus in tropical rain forests, but that the proportion of the total phosphorus in organic forms is relatively insensitive to variation in climate and soil properties. Further work is now required to assess the contribution of soil organic

phosphorus to the nutrition and diversity of plants in these species-rich ecosystems.”
“Purpose: To determine the optimal position and orientation of the coracoid bone graft for the Latarjet procedure

for recurrent instability in patients with recurrent anterior instability and high degrees of glenoid Rabusertib Cell Cycle inhibitor bone loss. Methods: A systematic review of the GDC-0973 molecular weight literature including the Cochrane Database of Systematic Reviews, the Cochrane Central Register of Controlled Trials, PubMed (1980-2012), and Medline (1980-2012) was conducted. The following search teams were used: glenoid bone graft, coracoid transfer, glenoid rim fracture, osseous glenoid defect, and Latarjet. Studies deemed appropriate for inclusion were then analyzed. Study data collected included level of evidence, patient demographic characteristics, preoperative variables, intraoperative findings, technique details, and postoperative recovery and complications where available. Results: The original search provided a total of 344 studies. A total of 334 studies were subsequently excluded because they were on an irrelevant topic, used an arthroscopic technique, or were not published in English or because they were review articles, leaving 10 studies eligible for inclusion. Given the different methods used in each of the studies included in the review, descriptive analysis

was performed. The selleckchem duration of follow-up ranged from 6 months to 14.3 years postoperatively. With the exception of 2 studies, all authors reported on recurrent shoulder instability after Latarjet reconstruction; the rate of recurrent anterior shoulder instability ranged from 0% to 8%. Overall patient satisfaction was listed in 4 studies, each of which reported good to excellent satisfaction rates of more than 90% at final follow-up. Conclusions: As noted in this review, the current literature on Latarjet outcomes consists mostly of retrospective Level IV case series. Although promising outcomes with regard to a low rate of recurrent instability have been seen with these reports, it should be noted that subtle variations in surgical technique, among other factors, may drastically impact the likelihood of glenohumeral degenerative changes arising in these patients. Level of Evidence: Level IV, systematic review of Level IV studies.

Gene expression profiling (GEP) and miRNA

expression profiling clearly differentiated pediatric DLBCL from BL, forming distinct clusters regardless of patient age. However, pathway analysis of GEP data identified minor differences between corresponding pediatric and adult tumors. Predominance (6:1) of the germinal center B-cell subtype to activated B-cell subtype was found among pediatric DLBCL. Two cases were molecularly classified as primary mediastinal B-cell lymphoma. We observed frequent abnormalities in 8q24 in pediatric DLBCL, including MYC rearrangement in 31% (5 of 16) and gain or amplification Rigosertib chemical structure in 50% (6 of 12) nonrearranged cases. MYC rearrangement was present in 96% (23 of 24) BL cases. Array-based CGH analysis identified abnormalities that are shared between adult and pediatric DLBCL (+12q15, +19q13, -6q), and abnormalities unique to the pediatric cases (-4p14, GSK2126458 order -19q13.32, +16p11.2), suggesting distinct pathogenetic mechanisms relative to age. Elucidation of the underlying target genes may provide insight into factors that modulate outcome and could provide potential novel therapeutic targets with less toxicity for pediatric patients with B-cell non-Hodgkin lymphoma. (Blood.

2012;119(16):3757-3766)”
“Background. Mitral valve (MV) surgical procedures in the elderly are associated with profound operative and long-term mortality. We report our experience and results for MV surgical procedures in the elderly, especially with regard to the influence of comorbidities.\n\nMethods. Our hospital database was assessed to identify all patients who underwent MV surgical procedures at the age

of 70 years and older between 1999 and 2009. The data were retrospectively analyzed.\n\nResults. A total of 2,503 patients operated on during this 10-year period were identified. In 97% of patients, mitral regurgitation (MR) was the primary indication for operation, followed by coronary artery disease in 41.6% and aortic valve stenosis in 21.3%. The 30-day mortality learn more rate was 3.1%, and the long-term survival at 5 years was 55.2% (95% confidence interval, 52.3% to 57.5%). Coronary artery bypass grafting was identified to be associated with inferior short-term and long-term survival. Numerous comorbidities significantly influenced long-term survival. The observed mortality was significantly lower than predicted by EuroSCORE (17.2%).\n\nConclusions. MV operations in the elderly can be performed with a low early mortality and promising long-term survival. However, our large series demonstrates that comorbidities are to be attributed as the real burden for successful treatment of elderly patients undergoing MV procedures. (Ann Thorac Surg 2012;94:1999-2004) (c) 2012 by The Society of Thoracic Surgeons”
“Aims: The objective of this study was to determine if survival of culturable Campylobacter jejuni outside the host was increased by entrapment in pre-established biofilms.